Optical coherence tomography (OCT) has been widely applied to the diagnosis of eye diseases during the past two decades. However, valid evaluation methods are still not available for the clinical OCT devices. In order to assess the axial resolution of the OCT system, standard model eyes with micro-scale multilayer structure have been designed and manufactured in this study. Mimicking a natural human eye, proper Titanium dioxide (TiO2) materials of particles with different concentrations were sel