This user guide serves as an instruction manual for the TruBio Bioprocess Control Software version 5.0 specific to ThermoFisher Bioreactors. The guide includes detailed information on how to navigate and utilize the software efficiently to control the bioprocess. It offers step-by-step instructions and helpful tips on how to tune and optimize bioreactors to achieve the desired outcomes. Other sections cover troubleshooting, maintenance, and frequently asked questions. We hope that this guide will prove to be an essential tool in achieving proficient bioprocessing with TruBio and ThermoFisher equipment.